Identification of chitinolytic bacteria isolated from shrimp pond sediment and characterization of their chitinase encoding gene

2nd International Symposium on Marine and Fisheries Research

地球科学;农业科学

Triwijayani, A.U.^1 ; Puspita, I.D.^1 ; Murwantoko, M.^1 ; Ustadi, U.^1

Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora Gd. A4, Bulaksumur Yogyakarta, Indonesia^1

关键词: Aeromonas sp;    Chitinases;    Encoding genes;    Molecular techniques;    Pseudomonas stutzeri;    Sequence analysis;    Serratia marcescens;    Shrimp ponds;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/139/1/012051/pdf
DOI  :  10.1088/1755-1315/139/1/012051

学科分类：农业科学（综合）

来源: IOP

【 摘 要 】

Chitinolytic bacteria are a group of bacteria owning enzymes that able to hydrolyze chitin. Previously, we isolated chitinolytic bacteria from shrimp pond sediment in Bantul, Yogyakarta, and obtained five isolates showing high chitinolytic index named as isolate PT1, PT2, PT5, PT6 and PB2. The aims of this study were to identify chitinolytic bacteria isolated from shrimp pond sediment and to characterize the chitinase encoding gene from each isolate. The molecular technique was performed by amplification of 16S rDNA, amplification of chitinase encoding gene and sequence analysis. Two chitinolytic bacteria of PT1 and PT2 were similar to Aeromonas bivalvium strain D15, PT5 to Pseudomonas stutzeri strain BD-2.2.1, PT6 to Serratia marcescens strain FZSF02 and PB2 to Streptomyces misionensis strain OsiRt-1. The comparison of chitinase encoding gene between three isolates with those in Gen Bank shows that PT1 had similar sequences with the chi1 gene in Aeromonas sp. 17m, PT2 with chi1 gene in A. caviae (CB101) and PT6 with chiB gene in S. Marcescens (BJL200).