Last step in the cooperative pathway for galactolipid synthesis in spinach leaves: Formation of monogalactosyldiacylglycerol with C18 polyunsaturated acyl groups at both carbon atoms of the glycerol

FEBS Letters

Dubacq, J.P.²  Kader, J.C.³  Escoffier, A.³  Trémolières, A.¹  Oursel, A.³ 

[1] Institut de Physiologie Végétale, CNRS, avenue de la Terrasse, 91190 Gif-sur-Yvette, France;Laboratoire de Botanique, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris France;Laboratoire de Physiologie Cellulaire, Université Pierre et Marie Curie, 4 place Jussieu, 75005 Paris, France

关键词: Phosphatidylcholine;    Diacylglycerol;    Galactolipid synthesis;    Chloroplast;    (Spinach);    C18/C16;    C18 at C1;    C16 at C2 molecular species of glycerolipids;    C18/C18;    C18 at C1;    C18 at C2 molecular species of glycerolipids;    CDP-choline;    cytidine diphosphocholine;    DGDG;    digalactosyldiacylglycerol;    FFA;    free fatty acids;    GLC;    gas-liquid chromatography;    HPLC;    high-performance liquid chromatography;    DAG;    diacylglycerol;    MGDG;    monogalactosyldiacylglycerol;    PC;    phosphatidylcholine;    PL;    polar lipids;    PLTP;    phospholipid transfer protein;    TLC;    thin-layer chromatography;    UDP-gal;    uridine diphosphogalactose;   

DOI  :  10.1016/0014-5793(87)80259-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this paper, the formation of monogalactosyldiacylglycerol (MGDG) with C18 polyunsaturated acyl groups at both positions of the glycerol from phosphatidylcholine (PC) of liposomes was demonstrated by a three-step procedure: spinach chloroplasts were first incubated with liposomes of PC-containing polyunsaturated fatty acids at both positions of the glycerol in the presence of phospholipid transfer protein. Chloroplasts were then incubated with phospholipase C in order to form 1,2-diacylglycerol (1,2-DAG) from PC integrated in the chloroplast envelope. Finally, washed chloroplasts were incubated with UDP-gal and MGDG was formed by galactosylation of 1,2-DAG.

【 授权许可】

Unknown