A Ratiometric Fluorescence Sensor for Phospholipase Based on A Novel Nanohybrid

2018 International Conference on Advanced Materials, Intelligent Manufacturing and Automation

材料科学;机械制造;运输工程

Liu, Siyu^1

College of Life and Health Sciences, Northeastern University, Shenyang

110000, China^1

关键词: Breast cancer cells;    Hydrothermal treatments;    Nano hybrids;    Phosphatidylcholine;    Phospholipase C;    Phospholipases;    Ratiometric fluorescence sensors;    Sodium gluconate;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/382/2/022055/pdf
DOI  :  10.1088/1757-899X/382/2/022055

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

A facile route to prepare fluorescent carbon dots was developed by hydrothermal treatment of sodium gluconate. We further reported the incoporation of doxorubicin molecules into the prepared carbon dots/phospholipid hybrids that was synthesized by a self-assembly of a phosphatidylcholine monolayer on the surface of carbon dots. And we demonstrated the fluorescence resonance energy transfer (FRET) process between the fluorescence carbon dots and doxorubicin in the nanohybrid. The phospholipase C can catalyze the hydrolysis of phospholipid, which disrupts the carbon dots-phospholipid-doxorubicin complex. Herein, the developed FRET system was utilized as a ratiometric fluorescence sensor for phospholipase C detection in aqueous and bioimaging in breast cancer cell.