Delayed activation of phospholipase D by gonadotropin‐releasing hormone in a clonal pituitary gonadotrope cell line (αT3‐1)

FEBS Letters

Naor, Zvi¹  Liscovitch, Mordechai²  Netiv, Einat¹ 

[1] Department of Biochemistry, George S. Wise Faculty of Life Science, Tel Aviv University, Ramat Aviv 69978, Israel;Department of Hormone Research, The Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel

关键词: Diacylglycerol;    GnRH;    Phosphatidylcholine;    Phosphatidic acid;    Protein kinase C;    Signal transduction;   

DOI  :  10.1016/0014-5793(91)81396-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Stimulation of cultured pituitary cells from a gonadotrope lineage (αT3-1) by the gonadotropin-releasing hormone agonist analog [D-Trp⁶]GnRH (GnRH-A) resulted in a manifold increase in accumulation of phosphatidylethanol, a specific product of phospholipase D phosphatidyl transferase activity when ethanol is the phosphatidyl group acceptor. Levels of the natural lipid product of phospholipase D, phosphatidic acid, were increased 2–3-fold. Activation of phospholipase D by GnRH-A was dose- and time-dependent and was blocked by a GnRH receptor antagonist [D-pClPhe²,D-Trp^3.6]GnRH. GnRH-A stimulated phospholipase D activity after a lag of 1–2 min. We conclude that in αT3-1 gonadotropes GnRH receptor occupancy results in delayed activation of phospholipase D which could participate in late phases of gonadotrope regulation by the neurohormone.

【 授权许可】

Unknown