Arginine‐vasopressin stimulates the formation of phosphatidic acid in rat Leydig cells

FEBS Letters

Jensen, Benny¹  Hansen, Harald S.¹  Nielsen, Jane R.¹ 

[1] Biochemical Laboratory, Royal Danish School of Pharmacy, Universitetsparken 2, 2100 Copenhagen Ø, Denmark

关键词: Arginine-vasopressin;    Phosphatidic acid;    V1 receptor;    Arachidonic acid;    Diacylglycerol;    (Leydig cell);    AVP;    arginine-vasopressin;    dPTyr(Me)AVP;    [1-deaminopenicillamine;    (O-methyl)Tyr2;    Arg8]vasopressin;    dPVDAVP;    [1-deaminopenicillamine;    Val4;    Arg8]vasopressin;    PI;    phosphatidylinositol;    PIP + P1P22;    phosphatidylinositol 4-phosphate + phosphatidylinositol 4;    5-bisphosphate;    DG;    diacylglycerol;    PA;    phosphatidic acid;   

DOI  :  10.1016/0014-5793(87)81025-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Arginine-vasopressin (AVP) stimulated the formation of labelled phosphatidic acid (PA) in [¹⁴C]arachidonic acid-prelabelled rat Leydig cells. After addition of 10^t̄6M AVP [¹⁴C]arachidonoylphosphatidic acid reached a maximum within 2 min. The increase was dose-dependent (10⁻¹¹−10⁻⁶ M). No change in labelling of other phospholipids and diacylglycerol could be detected. The V₁ antagonist dPTyr(Me)AVP inhibited in a dose-dependent manner the AVP-stimulated accumulation of PA. The V₂ agonist dPVDAVP was without effect. The present results suggest that AVP binds to V₁ receptors in rat Leydig cells resulting in stimulation of PA turnover. We suggest that the AVP-stimulated PA formation is an indication of phosphoinositide turnover.

【 授权许可】

Unknown