Enhancement of phospholipase A2 activation by phosphatidic acid endogenously formed through phospholipase D action in rat peritoneal mast cell

FEBS Letters

Akiba, Satoshi¹  Ishimoto, Tsuyoshi¹  Fujii, Tatsuzo¹  Sato, Takashi¹ 

[1] Department of Biochemistry, Kyoto Pharmaceutical University, Yamashina-ku, Kyoto 607, Japan

关键词: Phospholipase A2;    Phospholipase D;    Phosphatidic acid;    Arachidonic acid;    Rat peritoneal mast cell;    AA;    arachidonic acid;    DAG;    1;    2-diacylglycerol;    PA;    phosphatidic acid;    PL;    phospholipase;   

DOI  :  10.1016/0014-5793(93)81440-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Contribution of phosphatidic acid (PA) generated by activated phospholipase (PL) D to PLA₂ activation was studied in rat peritoneal mast cells. Exogenous didecanoyl PA induced arachidonate liberation in the permeabilized cells which was inhibited by p-bromophenacyl bromide. Upon exposure of the cells to ethanol in a high enough concentration to prevent PA formation, A23187-induced arachidonate liberation was suppressed by 50% and the rest was completely inhibited by p-bromophenacyl bromide. In contrast, propranolol, which enhanced PA accumulation, significantly increased the arachidonate liberation. These results suggest that A23187-induced PLA₂ activation may be potentiated, at least in part, by PA generated through PLD action.

【 授权许可】

Unknown