The phosphatidylcholine pathway of diacylglycerol formation stimulated by phorbol diesters occurs via phospholipase D activation

FEBS Letters

Cabot, Myles C.¹  Welsh, Clement J.¹  Cao, Hui-ting¹  Chabbott, Holly¹ 

[1] W. Alton Jones Cell Science Center, Lake Placid, NY 12946, USA

关键词: Phosphatidylcholine;    Diacylglycerol;    Phospholipase D;    Phorbol diester;    Vasopressin;    (REF52 cell);    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    DG;    diacylglycerol;    PKC;    Ca2+-activated;    phospholipid-dependent protein kinase C;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    PC;    phosphatidylcholine;    BSA;    bovine serum albumin;    VP;    vasopressin;    PA;    phosphatidate;    TLC;    thin-layer chromatography. In the naming of PC;    PA and DG we do not distinguish between the type of aliphatic linkage to glycerol (ester;    ether);   

DOI  :  10.1016/0014-5793(88)81374-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Agonist-induced degradation of phosphatidylcholine (PC) is of interest as this pathway of diacylglycerol (DG) generation may provide added opportunities for the regulation of protein kinase C (PKC). In REF52 cells [³H]myristic acid is preferentially incorporated into PC; this, coupled with the use of [³H]choline, allows for quantitation of both the water-soluble and the lipid products generated when PC is degraded. In cells prelabeled with [³H]choline, TPA stimulated a time-dependent release, into the medium, of choline and not phosphocholine or glycerophosphocholine. Treatment of [³H]myristic acid-labeled cells with either phorbol diesters, sn-1,2-dioctanoylglycerol, or vasopressin elicited the formation of labeled phosphatidate (PA) and DG. The temporal pattern of PC hydrolysis in cells treated with TPA is indicative of a precursor (PA)-product (DG) relationship for an enzymatic sequence initiated by phospholipase D. Adding propranolol, a phosphatidate phosphohydrolase inhibitor, eliminated TPA-induced DG formation, whereas PA generation was unaffected. From these data we conclude that TPA elicits DG formation from PC by the sequential actions of phospholipase D and phosphatidate phosphohydrolase.

【 授权许可】

Unknown