Catecholamine inhibition of Ca2+‐induced insulin secretion from electrically permeabilised islets of Langerhans

FEBS Letters

Fyles, Julie M.¹  Howell, Simon L.¹  Jones, Peter M.¹  Persaud, Shanta J.¹ 

[1] MRC Secretory Mechanisms Group, Department of Physiology, King's College London, Campden Hill Road, Kensington, London W8 7 AH, England

关键词: Insulin secretion;    Electrical permeabilisation;    Noradrenaline;    cyclic AMP;    (Islets of Langerhans);   

DOI  :  10.1016/0014-5793(87)81206-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Noradrenaline (1–10 μM) inhibited Ca²⁺-induced insulin secretion from electrically permeabilised islets of Langerhans with an efficacy similar to that for inhibition of glucose-induced insulin secretion from intact islets. The inhibition of insulin secretion from permeabilised islets was blocked by the α₂-adrenoreceptor antagonist, yohimbine. Adenosine 3′,5′-cyclic monophosphate (cAMP) did not relieve the noradrenaline inhibition of Ca²⁺-induced secretion from the permeabilised islets, although noradrenaline did not affect the secretory responses to cAMP at substimulatory (50 nM) concentrations of Ca²⁺. These results suggest that catecholamines do not inhibit insulin secretion solely by reducing B-cell adenylate cyclase activity, and imply that one site of action of noradrenaline is at a late stage in the secretory process.

【 授权许可】

Unknown