Regulation and specificity of glucose‐stimulated insulin gene expression in human islets of Langerhans

FEBS Letters

Ashcroft, Stephen J.H.³  Schofield, Paul N.¹  Hammonds, Peter³  Sutton, Robert²  Gray, Derek W.R.² 

[1] CRC Developmental Tumours Group, Department of Zoology, South Parks Rd, Oxford, England;Nuffield Department of Surgery, John Radcliffe Hospital, Headington, Oxford OX3 9DU, England;Nuffield Department of Clinical Biochemistry, John Radcliffe Hospital, Headington, Oxford OX3 9DU, England

关键词: Insulin gene expression;    Preproinsulin mRNA;    Insulin secretion;    cyclic AMP;    Protein kinase;    (Human pancreatic islet);   

DOI  :  10.1016/0014-5793(87)80523-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The insulin response of cultured human islets of Langerhans was measured at both mRNA and polypeptide levels in response to natural and pharmacological stimuli. We report a dosage dependent stimulation of both mRNA levels and insulin secretion by extracellular glucose, and present evidence that islet responsiveness can be divided into two temporal phases: an early response, apparently under post-transcriptional control, and a late phase in which insulin messenger accumulates. Although glucose effects in man are similar to rodents, there are important differences, especially with respect to modulation of glucose stimulation by activators of β-cell protein kinases.

【 授权许可】

Unknown