Parathyroid hormone regulates phosphate transport in OK cells via an irreversible inactivation of a membrane protein

FEBS Letters

Malmström, Kerstin¹  Murer, Heini¹ 

[1] Institute of Physiology, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland

关键词: Phosphate transport;    Parathyroid hormone;    Membrane transport;    Protein synthesis;    Cell culture;    (Proximal tubule);   

DOI  :  10.1016/0014-5793(87)80701-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In a cultured, renal epithelial cell line, OK, parathyroid hormone (PTH) reduces Na/phosphate cotransport and upon removal of the hormone the activity is regained. Cycloheximide, an inhibitor of protein synthesis, did not interfere with the PTH action, but prevented the reduced phosphate transport from regaining its original activity. Drugs, such as colchicine, that disrupt the microtubular network, lessened both the action of PTH and prevented the recovery of activity. The results are consistent with the irreversible inactivation of a plasma membrane protein necessary for full activity of the Na/phosphate cotransport.

【 授权许可】

Unknown