İron binding to microsomes and liposomes in relation to lipid peroxidation

FEBS Letters

Winterbourn, Christine C.¹  Vile, Glenn F.¹ 

[1] Department of Pathology, Christchurch School of Medicine, Christchurch Hospital, Christchurch, New Zealand

关键词: Lipid peroxidation;    Microsome;    Iron;    Chelator;   

DOI  :  10.1016/0014-5793(87)80131-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of ADP, ATP, citrate and EDTA on iron-dependent microsomal and liposomal lipid peroxidation, and on ⁵⁹FeCl₃ binding to the lipid membranes were measured. The aim was to test if initiation of lipid peroxidation is a site-specific mechanism requiring bound iron. In the absence of chelator, iron was bound to both membranes. EDTA and citrate removed the iron and inhibited peroxidation. ATP and ADP stimulated peroxidation, but whereas ADP allowed only half of the iron to remain bound, all was removed by ATP. Chelators, therefore, cannot be simply influencing a site-specific mechanism. Their effects must relate to the reactivities of the different iron chelates as initiators of lipid peroxidation.

【 授权许可】

Unknown