Enhancement of hydrogen peroxide‐induced luminol‐dependent chemiluminescence by neopterin depends on the presence of iron chelator complexes

FEBS Letters

Werner, Ernst R.¹  Esterbauer, Hermann²  Murr, Christian¹  Fuchs, Dietmar¹  Reibnegger, Gilbert¹  Gössler, Walter³  Werner-Felmayer, Gabriele¹  Wachter, Helmut¹  Hausen, Arno¹ 

[1] Institute for Medical Chemistry and Biochemistry, University of Innsbruck, Fritz Pregl Str. 3, A-6020 Innsbruck, Austria;Institute for Biochemistry, University of Graz, A-8010 Graz, Austria;Institute for Analytical Chemistry, University of Graz, A-8010 Graz, Austria

关键词: Neopterin;    Macrophage;    Hydrogen peroxide;    Iron;    Chelator;   

DOI  :  10.1016/0014-5793(94)80369-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously shown that neopterin, 6-d-erythro-trihydroxypropyl-pteridine, synthesized by human monocytes/macrophages upon stimulation by interferon-γ, enhances toxicity of reactive oxygen at neutral or slightly alkaline pH (7.5), but not at acidic pH (below 6.5). In the present study, we explored in more detail the necessary requirements for neopterin to modulate the effects of hydrogen peroxide in a luminol-dependent chemiluminescence assay. We demonstrate that neopterin enhances hydrogen peroxide effects only in the presence of iron chelator complexes like iron-(III)- or iron-(II)-EDTA or iron-(III)-DTPA. Thus, iron chelator complexes together with neopterin may play an important role in macrophage-mediated effector mechanisms.

【 授权许可】

Unknown