Co‐solubilization of asymmetric acetylcholinesterase and dermatan sulfate proteoglycan from the extracellular matrix of rat skeletal muscles

FEBS Letters

Brandan, Enrique¹  Inestrosa, Nibaldo C.¹ 

[1] Molecular Neurobiology Group, Department of Cell Biology, Catholic University of Chile, PO Box 114-D, Santiago, Chile

关键词: Acetylcholinesterase;    Heparin;    Dermatan sulfate proteoglycan;    (Muscle extracellular matrix);    AChE;    acetylcholinesterase;    HSPG;    heparan sulfate proteoglycan;    ECM;    extracellular matrix;    GAG;    glycosaminoglycan;    DSPG;    dermatan sulfate proteoglycan;   

DOI  :  10.1016/0014-5793(87)81483-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously communicated that heparin released asymmetric acetylcholinesterase (AChE) from cholinergic synapses. Here we report studies showing that heparin, besides releasing asymmetric AChE from the skeletal muscle extracellular matrix (ECM), specifically solubilizes a dermatan sulfate proteoglycan (DSPG) which accounts for more than 95% of the ³⁵S-released material. The co-solubilization of AChE and the proteoglycan opens up the possibility that both macromolecules could be involved in the formation of the soluble AChE complex observed after incubation of muscle homogenate with heparin. Our results suggest a possible association between asymmetric AChE and DSPG at the muscle ECM, moreover this work is the first report of the existence of DSPG at the skeletal muscle cell surface.

【 授权许可】

Unknown