Two‐dimensional lattices of porin diffract to 6 Å resolution

FEBS Letters

Büldt, Georg¹  Mischel, Maja¹  Rosenbusch, Jürg P.¹  Hentschel, Manfred P.¹  Regenass, Martin¹ 

[1] Department of Physics/Biophysics, Free University of Berlin, Arnimallee 14, Germany

关键词: Porin;    X-ray diffraction;    2D Crystal lattice;    (Escherichia coli);    Reconstitution;    Membrane protein;   

DOI  :  10.1016/0014-5793(86)80859-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Porin (the product of gene ompF) is an integral membrane protein (M _r 36 500) of the outer membrane of Escherichia coli (strain B^E). The protein has been purified to homogeneity and reconstituted in dimyristoyl-lecithin. Oriented specimen on a flat surface yielded X-ray diffraction pattern, originating from the two-dimensional protein lattice, to a resolution reaching 6 Å. Although these powder rings are broad compared to corresponding diffraction patterns from purple membranes of Halobacterium halobium, porin is the first reconstituted integral membrane protein which shows diffraction to this resolution.

【 授权许可】

Unknown