A patch‐clamp study of ion channels of inner and outer membranes and of contact zones of E. coli, fused into giant liposomes

FEBS Letters

Houssin, Christine²  Coulombe, Alain¹  Ghazi, Alexandre²  Berrier, Catherine² 

[1] Laboratoire de Physiologie Comparée, URA CNRS 1121, Université Paris-Sud, 91405, Orsay Cedex, France;Laboratoire des Biomembranes, URA CNRS 1116 Université Paris-Sud, 91405, Orsay Cedex, France

关键词: Ion channel;    Porin;    Pressure activation;    Patch-clamp;    (Escherichia coli);   

DOI  :  10.1016/0014-5793(89)81486-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Inner and outer membranes of Escherichia coli and contact zones were isolated and fused separately with giant liposomes amenable to patch-clamp recording. Different types of large pressure-activated channels were localized in the inner membrane fraction which also contained smaller, pressure-insensitive channels. The outer membrane contained pressure-insensitive channels with large conductances and long opening and closing times which are likely to be porins. Large channels were also observed in contact zones.

【 授权许可】

Unknown