Membrane‐derived oligosaccharides (MDO's) promote closing of an E. coli porin channel

FEBS Letters

Delcour, Anne H.¹  Kung, Ching²  Adler, Julius¹  Martinac, Boris² 

[1] Department of Biochemistry and Genetics. University of Wisconsin-Madison, Madison, WI 53706, USA;Laboratory of Molecular Biology and Department of Genetics, University of Wisconsin-Madison, Madison, WI 53705, USA

关键词: Porin;    Membrane-derived ollgosaccharide;    Ion channel;    Outer membrane;    Patch-clamp;    Esrcherichia coli;   

DOI  :  10.1016/0014-5793(92)80622-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The outer membrane or Escherichia coli is a diffusion barrier for macromolecules, but allows the passage of small hydrophilic solutes through non-specific channels, the porins. Some electrophysiological studies find reconstituted porins in a mostly open state, while those done with the patch-clamp technique performed on live cells suggest that the vast majority of the native channels are closed. We present here current measurements through porins from reconstituted outer membrane, which demonstrate that bacterial metabolites, the MDO's, which bathe the periplasmic side of the outer membrane, induce the channels to close. These findings illustrate that the degree or openess of porins can be regulated by compounds naturally found in bacteria.

【 授权许可】

Unknown