Cloning of the protein D2 gene of Pseudomonas aeruginosa and its functional expression in the imipenem‐resistant host

FEBS Letters

Yoneyama, Hiroshi¹  Nakac, Taiji¹ 

[1] Department of Cellular Information Sciences, Tokai University, School of Medicine, Isehara 259-11, Japan

关键词: Pseudomonas aeruginosa;    Protein D2;    Imipenem;    Permeability;    Porin;    Outer membrane;    D2;    protein D2;    CBPC;    carbenicillin;    ABPC;    ampicillin;    KM;    kanamycin;    MIC;    minimum inhibitory concentration;   

DOI  :  10.1016/0014-5793(91)80582-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein D2 forms the water-filled pore across the outer membrane of Pseudomonas aeruginosa and allows the penetration of imipenem. We cloned the protein D2 gene by the antibody screening technique. When the imipenem-resistant mutant lacking protein D2 harbored the plasmid with the cloned D2 gene, the mutant overproduced protein D2 in the outer membrane. These transformants exhibited fully-restored imipenem susceptibility. The results prove unequivocally that protein D2 forms the imipenem-permeable pore in the P. aeruginosa outer membrane.

【 授权许可】

Unknown