Ion‐conducting channels in a Gram‐positive bacterium

FEBS Letters

Zoratti, Mario¹  Petronilli, Valeria¹ 

[1] CNR Unit for the Physiology of Mitochondria, Institute of General Pathology, Via Loredan 16, Padova, Italy

关键词: Ion channel;    Patch clamp;    Pressure activation;    Conductance substate;    (Gram-positive bacteria;    Streptococcus faecalis);   

DOI  :  10.1016/0014-5793(88)80348-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The patch-clamp technique was used to obtain information on the existence and properties of ion channels in giant protoplasts obtained from the Gram-positive bacterium Streptococcus faecalis. The membrane proved to contain a pore with numerous conductance states, ranging from 10 pS to several nanosiemens. Application of a slight pressure differential across the membrane resulted in the activation of the channel. The pressure sensitivity points to a relationship between this channel and one recently discovered in E.coli spheroplasts [(1987) Proc. Natl. Acad. Sci. USA 84, 2297–2301] suggesting that pores of this type might be widespread among prokaryotes.

【 授权许可】

Unknown