The effects of 1‐aminooxy‐3‐aminopropane and S‐(5'‐deoxy‐5'‐adenosyl)methylthioethylhydroxylamine on ornithine decarboxylase and S‐adenosyl‐L‐methionine decarboxylase from Escherichia coli

FEBS Letters

Paulin, Lars¹ 

[1] Department of Pharmacology and Toxicology, College of Veterinary Medicine, PO Box 6, Hämeentie 57, SF-00551 Helsinki, Finland

关键词: Ornithine decarboxylase;    S-Adenosyl-L-methionine decarboxylase;    Enzyme inhibition;    (Escherichia coli);   

DOI  :  10.1016/0014-5793(86)80710-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

1-Aminooxy-3-aminopropane (APA) was shown to be a potent competitive inhibitor (K _i = 1.0 nM) of partially purified Escherichia coli ornithine decarboxylase. APA did not inhibit S-adenosyl-L-methionine decarboxylase and spermidine synthase from E. coli. S-(5'-Deoxy-5'-adenosyl)methylthioethylhydroxylamine (AMA), which is a structural analogue of decarboxylated S-adenosyl-L-methionine, was for the first time shown to be an irreversible inhibitor of bacterial S-adenosyl-L-methionine decarboxylase and a competitive inhibitor (K _i = 47 μM) of bacterial ornithine decarboxylase. AMA had no effect on spermidine synthase.

【 授权许可】

Unknown