Synthesis of paf‐acether from exogenous precursors by the prokaryote Escherichia coli

FEBS Letters

Bossant, Marie-Jeanne²  Kim, Hee-Yong¹  Thomas, Yolène²  Dassa, Elie³  Benveniste, Jacques²  Salem, Norman¹  Denizot, Yves² 

[1] National Institute on Alcohol Abuse and Alcoholism, DICBR, Laboratory of Clinical Studies, Bethesda, MD 20892, USA;Institut National de la Santé et de la Recherche Médicale, U 200, Université Paris-Sud, 32 rue des Carnets, 92140 Clamart, France;Institut National de la Santé et de la Recherche Médicale, U 163, Unité de programmation moléculaire et toxicologie génétique, CNRS, UA 171, Institut Pasteur, 28 rue du Docteur Roux, 75015 Paris, France

关键词: Platelet activating factor-acether;    Mass spectral analysis;    (Escherichia coli);   

DOI  :  10.1016/0014-5793(89)81207-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Paf-acether (paf) is a potent mediator of inflammatory diseases and septic shock. Using normal-phase HPLC, a paf-like activity was found in culture supernatants from E. coli. Prokaryotic paf exhibited the same biological and physico-chemical properties as eukaryotic cells and synthetic paf. Further, reverse-phase HPLC indicates that paf generated by bacteria is predominantly of the hexadecyl and octadecyl species. When cultures were supplemented with lyso-paf, a dramatic increase in paf production was observed. The purity and molecular structure of bacterial paf were further characterized by mass spectral analysis. These results could be of importance considering the pathogenetic role of enterobacteria. Further, it appears that the competence to form and release paf is an early phylogenetic development.

【 授权许可】

Unknown