cDNA clones coding for the β‐subunit of human liver alcohol dehydrogenase have differently sized 3'‐non‐coding regions

FEBS Letters

von Bahr-Lindström, Hedvig¹  Heden, Lars-Olof¹  Larsson, Kerstin¹  Lagerholm, Eva¹  Höög, Jan-Olov¹  Vallee, Bert L.¹  Holmgren, Arne¹  Lake, Mats¹  Jörnvall, Hans¹ 

[1] Department of Chemistry I, Karolinska Institutet S-104 01 Stockholm, KabiGen AB, S-112 87 Stockholm, Sweden and Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, MA 02115, USA

关键词: Alcohol dehydrogenase;    cDNA;    Oligonucleotide probe;    DNA sequence;   

DOI  :  10.1016/0014-5793(86)80111-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Three different size classes of cDNA clones coding for the β₁-rsubunit of human alcohol dehydrogenase (ADH) were characterized from a human liver cDNA library. Clones were identified by hybridization with synthetic oligodeoxyribonucleotides. A total of 2530 nucleotides were determined, covering an ADH-coding region of 1122 nucleotides, a preceding 72-nucleotide segment and 3 types of 3'-non-coding region. The coding nucleotide sequence is in full agreement with the amino acid sequence of the β₁subunit. Of 8 clones identified, 6 had a short, 213-nucleotide 3'-non-coding region; 1 an intermediate, 590-nucleotide 3'-region; and 1 a long, 1330-nucleotide 3'-region. In addition, 2 unused polyadenylation signals were found. These results suggest that human liver β-ADH mRNAs occur in several size classes, and that in addition to the consensus sequence AATAAA further signals are important for 3'-end formation.

【 授权许可】

Unknown