cDNA cloning and nucleotide sequence of lipocortin‐like 33 kDa protein in guinea pig neutrophils

FEBS Letters

Utsumi, Kozo²  Sato, Eisuke F.²  Tanaka, Yoshikazu¹ 

[1] Institute for Fundamental Research Center, Suntory, Ltd, Shimamoto-cho, Mishima-gun, Osaka 618, Japan;Department of Medical Biology, Kochi Medical School, Nankoku-shi, Kochi 781-51, Japan

关键词: Protein;    33 kDa;    Lipocortin;    cDNA;    DNA sequence;    cDNA;    complementary DNA;    p33;    33 kDa protein from guinea pig neutrophils;    PLA2;    phospholipase A2;    PKC;    Ca2+- and phospholipid-dependent protein kinase;    [α-32P]dCTP;    deoxycytidine 5′-[α-32P]triphosphate;    [γ-32P]-ATP;    adenosine 5′-[γ-32P]triphosphate;    SSC;    sodium chloride and sodium citrate buffer;   

DOI  :  10.1016/0014-5793(89)81173-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

cDNA clones of guinea pig neutrophil 33 kDa protein, a lipocortin like-protein, were isolated from two λgt10 libraries and one primer-extended λgt10 library of guinea pig neutrophils using synthetic oligonucleotide probes or cDNA fragment probe. The cDNA consists of 1389 nucleotides, and contains 1038 nucleotides encoding 346 amino acids of 33 kDa protein and a poly(A) tail. The deduced amino acid sequence shows high homology with those of lipocortin 1 from human U937 cells (89% homology) and rat lung (86%).

【 授权许可】

Unknown