Expression of the cDNA encoding lipocortin‐like 39 kDa protein of guinea pig neutrophils in yeast

FEBS Letters

Utsumi, Kozo²  Inoue, Masayasu³  Morishita, Seiji²  Edashige, Keisuke²  Sato, Eisuke F.²  Kobuchi, Hirotsugu²  Sugino, Yasuo M.²  Tanaka, Yoshikazu¹ 

[1] Institute for Fundamental Research, Research Center, Suntory, Ltd, Shimamoto-cho, Mishima-gun, Osaka 618, Japan;Department of Medical Biology, Kochi Medical School, Nankoku-shi, Kochi 781-51, Japan;Department of Biochemistry, Faculty of Medicine, Kumamoto University, Honjo, Kumamoto 860, Japan

关键词: Lipocortin;    Recombinant protein;    Neutrophil;    Protein kinase;    cDNA;    complementary DNA;    39K;    39 kDa protein;    PLA2;    phospholipase A2;    PKC;    Ca2+- and phospholipid-dependent protein kinase;    EGF;    epidermal growth factor;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(89)81097-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cDNA encoding lipocortin-like 39 kDa protein in guinea pig neutrophils was cloned into a yeast expression vector and the constructed plasmid was introduced into a yeast. The gene was expressed in an eukaryotic cell, yeast Saccharomyces cerevisiae and the recombinant protein was purified and characterized. The purified protein was identical with the native one with respect to the antigenicity and several biochemical properties, such as inhibitory action against phospholipase A₂, Ca²⁺-dependent binding to acidic-phospholipids and F-actin and availability as a substrate for tyrosine kinase (EGF receptor/kinase) and protein kinase C.

【 授权许可】

Unknown