Tyrosine kinase activity is associated with CD44 in human neutrophils

FEBS Letters

Skubitz, Amy P.N²  Skubitz, Keith M³  Campbell, Kenneth D¹ 

[1] Masonic Cancer Center, Minneapolis, MN 55455, USA;Laboratory of Medicine and Pathology, University of Minnesota Medical School, Minneapolis, MN 55455, USA;Department of Medicine, University of Minnesota Medical School, Box 325, University Hospital, Minneapolis, MN 55455, USA

关键词: Signal transduction;    Protein kinase;    Neutrophil;    CD44;    Inflammation;    Phagocyte;    DFP;    diisopropylfluorophosphate;    NMS;    normal mouse serum;    NRS;    normal rat serum;    NaCl-HEPES;    145 mM NaCl;    20 mM HEPES;    pH 7.3;    Brij solubilization buffer;    20 mM Tris-HCl;    pH 8.2;    150 mM NaCl;    1 mM PMSF;    2 mM MgCl2;    0.02% NaN3;    and 1.0% Brij 58;    Brij wash buffer;    20 mM Tris-HCl;    pH 8.2;    150 mM NaCl;    1 mg/ml BSA;    0.5% Brij 58;    2 mM MgCl2;    0.125 mg/ml gelatin;    1 mM PMSF;    and 0.02% NaN3;    Labeling buffer;    NaCl-HEPES;    0.1% Brij 58;    6 mM MnCl2;    40 mM MgCl2;    200 μM Na3VO4;    200 μM Na2MoO4;    and 10 μCi of [γ-32P]ATP;    Buffer A;    1 mg/ml BSA;    0.05% NP-40;    20 mM Tris-HCl;    pH 7.6;    100 mM NaCl;    1 mM EDTA;    0.05% NaN3;    Brij-SA buffer;    1 mg/ml BSA;    0.05% Brij 58;    20 mM Tris-HCl;    pH 7.6;    100 mM NaCl;    1 mM EDTA;    0.05% NaN3;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(98)01347-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

CD44 appears to be involved in signal transduction, however, the mechanism of this function is unclear. Protein kinase activity was detected in neutrophils associated with CD44. Most of the protein kinase activity associated with these antigens was tyrosine kinase activity. Src family kinases lyn and hck were found to account for much of the associated tyrosine kinase activity. The data suggest that associated tyrosine kinase activity may play a role in signal transduction from CD44 in neutrophils to regulate other cell functions.

【 授权许可】

Unknown