Synthetic peptide from lipocortin I has no phospholipase A2 inhibitory activity

FEBS Letters

van Binsbergen, J.¹  de Haas, G.H.¹  Aarsman, A.J.¹  Slotboom, A.J.¹ 

[1] Centre for Biomembranes and Lipid Enzymology, Padualaan 8, PO Box 80054, 3508 TB Utrecht, The Netherlands

关键词: Lipocortin;    Phospholipase A2;    Synthetic peptide;    PLA2;    phospholipase A2;    t-Boc;    tert. butyloxycarbonyl;    O-But;    tert. butylester;    Cl2-Z;    dichloro-benzyloxycarbonyl;    DOC;    sodium deoxycholate;    CMC;    critical micellar concentration;    HPLC;    high-performance liquid chromatography;   

DOI  :  10.1016/0014-5793(89)81355-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two anti-inflammatory peptides corresponding to a high amino acid similarity region between lipocortins were synthesized and tested on their ability to inhibit porcine pancreatic phospholipase A₂. Kinetic assays using monomeric and aggregated phospholipids did not reveal any phospholipase A₂ inhibitory activity. The peptides did not inhibit phospholipase A₂ activity on monolayers of negatively charged substrate and did not prevent phospholipase A₂ action on mixed micelles of 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and sodiumdeoxycholate. Ultraviolet difference spectroscopy did not show binding of the peptides to phospholipase A₂. Therefore we conclude that these anti-inflammatory peptides do not inhibit pancreatic phospholipase A₂ in vitro, in contrast to the results recently published [(1988) Nature 335,726–730].

【 授权许可】

Unknown