Selective modification of cytoplasmic membrane fluidity by catalytic hydrogenation provides evidence on its primary role in chilling susceptibility of the blue‐green alga, Anacystis nidulans

FEBS Letters

Joó, Ferenc²  Vigh, László³  Gombos, Zoltán¹ 

[1] Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, 6701 SzegedHungary;Institute of Physical Chemistry, Kossuth Lajos University, PO Box 7, 4010 Debrecen, Hungary;Institute of Biochemistry, Hungarian Academy of Sciences, PO Box 521, 6701 SzegedHungary

关键词: Catalytic hydrogenation;    Blue-green alga;    Cytoplasmic membrane;    Chilling susceptibility;   

DOI  :  10.1016/0014-5793(85)80008-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cytoplasmic and thylakoid membranes were isolated at different stages of catalytic hydrogenation of fatty acyl double bonds in living cells of Anacystis nidulans. The reaction was limited to the lipids of cell-surface membranes during the initial period of hydrogenation, thus, we were able to produce alga cells, modified exclusively in the cytoplasmic membrane. In these unique cells, neither compositional nor physical changes were detected in the lipid phase of thylakoids. Due to the rigidification of the alkyl chains of its lipids, however, the cytoplasmic membrane became leaky and phase separated at considerably higher chilling temperatures.Since an irreversible inactivation of photosynthetic electron transport was found simultaneously, it provided direct evidence for the hypothesis that the thermotropic properties of the lipids within cytoplasmic membranes, and not those of the thylakoids, control chilling susceptibility of the blue-green alga, A.nidulans.

【 授权许可】

Unknown