In vivo study of the state of order of the membranes of Gram‐negative bacteria by Fourier‐transform infrared spectroscopy (FT‐IR)

FEBS Letters

Naumann, Dieter¹  Schultz, Christian¹ 

[1] Department of Cytology, Robert Koch-Institute, Nordufer 20, D-1000 Berlin 65, Germany

关键词: Fourier transform infrared spectroscopy (FT-IR);    Gas chromatography;    Cytoplasmic membrane;    Outer membrane;    Bacteria;    In vivo spectroscopy;   

DOI  :  10.1016/0014-5793(91)81339-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Temperature-induced order/disorder transition profiles were obtained from the membranes of intact Gram-negative bacterial cells by FT-IR analysis of the frequency shifts of the acyl chain methylene symmetric stretching band as a monitor. Cells grown at different temperatures yielded distinct transition profiles. At the individual growth temperatures, however, the nearly alike frequency values indicated a very similar ‘state of order’ of the bacterial membranes. The FT-IR data were complemented by GC analysis of whole cell fatty acid composition. The FT-IR data obtained in vivo gave direct evidence of the adaptation of the ‘state of order’ and ‘fluidity’ of bacterial membranes to varying growth temperatures.

【 授权许可】

Unknown