pH‐jump‐induced phosphorylation of ADP in the cyanobacterium Anacystis nidulans

FEBS Letters

Peschek, Günter A.¹  Muchl, Robert¹ 

[1] Biophysical Chemistry Group, Institute of Physical Chemistry, University of Vienna, Währingerstraβe 42, A-1090 Vienna, Austria

关键词: pH gradient;    Protonmotive force;    Reversible H+-ATPase;    ATP synthesis;    Cytoplasmic membrane;    Anacystis nidulans;   

DOI  :  10.1016/0014-5793(83)80031-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The low ATP levels in dark anaerobic cells of the cyanobacterium Anacystis nidulans more than doubled within 5 s after rapid addition of HCl shifting external pH from 9.0 to 4.5. Steady-state levels of ATP and intracellular pH remained constant at 0.95 ± 0.15 nmol/mg dry weight and 6.9 ± 0.3, respectively. ΔpH-induced ATP synthesis was inhibited by dicyclohexylcarbodiimide and carbonyl cyanide m-chlorophenylhydrazone but not by carbon monoxide. According to our results the cytoplasmic membrane of A. nidulans has to be regarded as an energy-transducing membrane bioenergetically similar to the thylakoid membrane.

【 授权许可】

Unknown