Mitochondrial ATPase activity and membrane fluidity changes in rat liver in response to intoxication with Buckthorn ( Karwinskia humboldtiana )

Biological research: BR

rez-Anguiano¹  Margarita Cid-Herná¹  rez²  nchez²  Ana C Ramí³  Eddic W Morales-Sá³  Sandra F Velasco-Ramí⁴  s⁴  Genaro G Ortiz⁵  ndez⁵  lez-Ortiz⁶  n P Pacheco-Moisé⁶  Fermí⁷  Luis J Gonzá⁷ 

[1] Departamento de QuíDivisióas, Universidad de Guadalajara, Guadalajara, Mexico;dica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Mexico;mica, Centro Universitario de Ciencias Exactas e Ingenierín Biomén de Neurociencias, Laboratorio de Desarrollo, Envejecimiento y Enfermedades Neurodegenerativas, Centro de Investigació

关键词: Karwinskia humboldtiana;    ATPase;    Mitochondria;    Membrane fluidity;    pH gradient;   

DOI  :  10.1186/s40659-015-0008-9

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Karwinskia humboldtiana (Kh) is a poisonous plant of the rhamnacea family. To elucidate some of the subcellular effects of Kh toxicity, membrane fluidity and ATPase activities as hydrolytic and as proton-pumping activity were assessed in rat liver submitochondrial particles. Rats were randomly assigned into control non-treated group and groups that received 1, 1.5 and 2 g/Kg body weight of dry powder of Kh fruit, respectively. Rats were euthanized at day 1 and 7 after treatment. Rats under Kh treatment at all dose levels tested, does not developed any neurologic symptoms. However, we detected alterations in membrane fluidity and ATPase activity. Lower dose of Kh on day 1 after treatment induced higher mitochondrial membrane fluidity than control group. This change was strongly correlated with increased ATPase activity and pH gradient driven by ATP hydrolysis. On the other hand, membrane fluidity was hardly affected on day 7 after treatment with Kh. Surprisingly, the pH gradient driven by ATPase activity was significantly higher than controls despite an diminution of the hydrolytic activity of ATPase. The changes in ATPase activity and pH gradient driven by ATPase activity suggest an adaptive condition whereby the fluidity of the membrane is altered.

【 授权许可】

CC BY