Temperature‐dependent conformational changes in isolated oligomycin‐sensitive ATPase

FEBS Letters

Lenaz, Giorgio²  Baracca, Alessandra³  Solaini, Giancarlo³  Curatola, Giovanna¹  Castelli, Giovanna Parenti³  Fiorini, Rosa Maria¹ 

[1] Istituto di Biochimica, University of Ancona, 60100 Ancona, Italy;Istituto Botanico, University of Bologna, Via Imerio 42, 40126 Bologna, Italy;Istituto di Chimica Biologica, University of Bologna, 40126 Bologna, Italy

关键词: ATPase;    Mitochondria;    Conformational change;    Kinetics;    Temperature effect;    Lipid role;    Membrane circular dichroism;   

DOI  :  10.1016/0014-5793(83)80224-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Isolated oligomycin-sensitive ATPase undergoes a kinetic change at 20–25°C with a higher activation energy and a lower K _m for ATP below this temperature range. This observation has been correlated with temperature-dependent structural changes detected by circular dichroism in the UV region in the isolated enzyme. The negative ellipticities in the 208-225 nm region, which are proportional to the α-helix content, increase with rise in temperature to a maximum above 25°C.

【 授权许可】

Unknown