In vivo 31P nuclear magnetic resonance saturation transfer measurements of phosphate exchange reactions in the yeast Saccharomyces cerevisiae

FEBS Letters

Campbell, Sharon L.¹  Shulman, Robert G.¹  Jones, Kerri A.¹ 

[1] Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511, USA

关键词: ATPase;    Kinetics;    Saturation transfer;    31P-NMR;    (Saccharomyces cerevisiae);    Metabolism;    rf;    radiofrequency;    NTP-γ;    γ-phosphate of nucleoside triphosphates;    NDPβ;    β-phosphate of nucleoside diphosphates;    NDPα- and NTPα;    α-phosphates of nucleoside di- and triphosphates;    NTPβ;    β-phosphate of nucleoside triphosphates;    NADP(H);    oxidized and reduced form of nicotinamide adenine dinucleotide phosphate;    PP1;    terminal phosphate of polyphosphate;    PP2 and PP3;    penultimate phosphates of polyphosphate;   

DOI  :  10.1016/0014-5793(85)80148-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

³¹P saturation transfer techniques have been used to measure phosphate kinetics in the yeast Saccharomyces cerevisiae. The phosphate consumption rate observed in acetate grown mid-log cells was combined with measurements of O₂ consumption to yield P/O ratios of 2.2 and 2.9, for cells respiring on glucose and ethanol, respectively. However, no phosphate consumption activity was observed in saturation transfer experiments on anaerobic glucose fed cells. The phosphate consumption rates measured by saturation transfer in cells respiring on glucose and ethanol was attributed to the unidirectional rates of mitochondria! ATP synthesis.

【 授权许可】

Unknown