31P NMR measurement of ATP synthesis rate in perfused intact rat hearts

FEBS Letters

Petein, M.²  Foker, J.E.³  Kingsley-Hickman, P.¹  Sako, E.Y.³  Ugurbil, K.¹  From, A.H.L.²  St. Cyr, J.A.³  Andreone, P.A.³  Michurski, S.¹ 

[1] >Departments of Biochemistry and Gray Freshwater Biological Institute, University of Minnesota, Navarre, MN 55392, USA;Cardiovascular Division, Department of Medicine, Minneapolis VA Medical Center and the University of Minnesota, Minneapolis, MN 55455, USA;Department of Surgery. University of Minnesota, Minneapolis, MN 55455, USA

关键词: 31P-NMR;    Saturation transfer;    ATPase;    ( Rat myocardium);   

DOI  :  10.1016/0014-5793(86)81204-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using ³¹P NMR and the saturation-transfer method, the unidirectional rate of ATP synthesis was measured in isolated, Langendorff-perfused, isovolumic rat hearts operating at a rate pressure product of 25.6 ± 2.5 (SE) × 10³ mmHg · min⁻¹ and consuming O₂; at a rate of 35 ± 2 μmol O₂ · min⁻¹. (g dry wt)⁻¹, at 37°C. This rate was 7.2 ± 0.9 μmol · s⁻¹ · (g dry wt)⁻¹ and was related to the rate of oxygen atom consumption by a ratio of 6.3 ± 0.9. These date show that in the intact heart the unidirectional rate of ATP synthesis exceeds the net rate of ATP synthesis and consumption by approximately a factor of 2.

【 授权许可】

Unknown