期刊论文详细信息
FEBS Letters
31P NMR measurement of ATP synthesis rate in perfused intact rat hearts
Petein, M.2  Foker, J.E.3  Kingsley-Hickman, P.1  Sako, E.Y.3  Ugurbil, K.1  From, A.H.L.2  St. Cyr, J.A.3  Andreone, P.A.3  Michurski, S.1 
[1] >Departments of Biochemistry and Gray Freshwater Biological Institute, University of Minnesota, Navarre, MN 55392, USA;Cardiovascular Division, Department of Medicine, Minneapolis VA Medical Center and the University of Minnesota, Minneapolis, MN 55455, USA;Department of Surgery. University of Minnesota, Minneapolis, MN 55455, USA
关键词: 31P-NMR;    Saturation transfer;    ATPase;    ( Rat myocardium);   
DOI  :  10.1016/0014-5793(86)81204-2
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Using 31P NMR and the saturation-transfer method, the unidirectional rate of ATP synthesis was measured in isolated, Langendorff-perfused, isovolumic rat hearts operating at a rate pressure product of 25.6 ± 2.5 (SE) × 103 mmHg · min−1 and consuming O2; at a rate of 35 ± 2 μmol O2 · min−1. (g dry wt)−1, at 37°C. This rate was 7.2 ± 0.9 μmol · s−1 · (g dry wt)−1 and was related to the rate of oxygen atom consumption by a ratio of 6.3 ± 0.9. These date show that in the intact heart the unidirectional rate of ATP synthesis exceeds the net rate of ATP synthesis and consumption by approximately a factor of 2.

【 授权许可】

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