Kinetic characterization of the acid—alkaline transition in Dolabella auricularia ferric myoglobin using 1H‐NMR saturation transfer experiments

FEBS Letters

Chûjô, Riichirô²  Suzuki, Tomohiko¹  Inoue, Yoshio²  Yamamoto, Yasuhiko² 

[1] Department of Biology, Faculty of Science, Kochi University, Kochi 780, Japan;Department of Biomolecular Engineering, Tokyo Institute of Technology, Meguro-ku, Tokyo 152, Japan

关键词: NMR;    Myoglobin;    Saturation transfer;    Acid—alkaline transition;    Hyperfine shift;   

DOI  :  10.1016/0014-5793(92)81149-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The acid—alkaline transition in ferric myoglobin of the mollusc, Dolabella auricularia, exerts the changes in both the coordination and spin states of the heme iron. Slower transition rate, compared to the NMR time scale, in this myoglobin allowed the observation of separate signals arising from the two forms, and pH tiltration yielded a pK value of 7.8. ¹H-NMR saturation transfer experiments have been successfully used not only to provide the first signal assignments for the heme methyl proton resonances of the Met-hydroxyl form of the myoglobin, but also to detemine the kinetics of the transition.

【 授权许可】

Unknown