Measuring the diffusion coefficient of ganglioside on cell membrane by fluorescence correlation spectroscopy

6th conference on Advances in Optoelectronics and Micro/nano-optics

Dong, Shiqing^1 ; You, Minghai^1 ; Chen, Jianling^1 ; Zhou, Jie^1 ; Xie, Shusen^1 ; Yang, Hongqin^1

Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology, Medicine of Ministry of Education, Fujian Normal University, Fuzhou

350007, China^1

关键词: Cholera toxin B subunit;    Cytoplasmic membrane;    Dynamic characteristics;    Fluorescence Correlation Spectroscopy;    gangliosides;    HeLa cell;    Living cell membranes;    Physiological functions;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/844/1/012047/pdf
DOI  :  10.1088/1742-6596/844/1/012047

来源: IOP

【 摘 要 】

The fluidity of proteins and lipids on cell membrane plays an important role in cell's physiological functions. Fluorescence correlation spectroscopy (FCS) is an effective technique to detect the rapid dynamic behaviors of proteins and/or lipids in living cells. In this study, we used the rhodamine6G solution to optimize the FCS system. And, cholera toxin B subunit (CT-B) was used to label ganglioside on living Hela cell membranes. The diffusion time and coefficients of ganglioside can be obtained through fitting the autocorrelation curve based on the model of two-dimensional cell membrane. The results showed that the diffusion coefficients of ganglioside distributed within a wide range. It revealed the lateral diffusion of lipids on cell membrane was inhomogeneous, which was due to different microstructures of cytoplasmic membrane. The study provides a helpful method for further studying the dynamic characteristics of proteins and lipids molecules on living cell membrane.