Pertussis toxin facilitates the progesterone‐induced maturation of Xenopus oocyte

FEBS Letters

Alouf, Joseph E.¹  Megret, Françoise¹  Mulner, Odile²  Ozon, René² 

[1] Unité des Antigènes Bactériens, ERA CNRS 794, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France;Laboratoire de Physiologie de la Reproduction, Groupe Stéroïdes, ERA CNRS 694, Université Pierre et Marie Curie, 4, Place Jussieu, 75230 Paris Cedex 05, France

关键词: Oocyte;    Progesterone;    Pertussis toxin;    Phosphorylation;   

DOI  :  10.1016/0014-5793(85)80300-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Progesterone triggers the first meiotic cell division of Xenopus oocyte and inhibits cAMP synthesis. The effect of pertussis toxin purified from Bordetella pertussis was tested on the maturation of Xenopus oocyte. The toxin did not inhibit progesterone-induced resumption of meiosis or the hormone-induced drop in cAMP level. This indicates that progesterone action is not mediated by the N_i subunit of the oocyte adenylate cyclase. Furthermore, pertussis toxin caused a reduction in the time course of maturation correlated with the precocious appearance of an alkali stable 47kDa phosphoprotein, a marker of the maturation promoting factor (MPF) activity. Pertussis toxin effects mimicked those of 2-glycerophosphate suggesting that both agents act on the steady-state level of phosphorylation implicated in MPF activity.

【 授权许可】

Unknown