Oocyte adenylyl cyclase contains Ni, yet the guanine nucleotide‐dependent inhibition by progesterone is not sensitive to pertussis toxin

FEBS Letters

Olate, Juan¹  Birnbaumer, Lutz¹  Sekura, Ronald D.¹  Allende, Catherine C.¹  Allende, Jorge E.¹ 

[1] Departamento de Bioquimica, Facultad de Medicina, and Departamento de Biologia, Facultad de Ciencias Basicas y Farmaceuticas, Universidad de Chile, Casilla 6671, Santiago 7, Chile

关键词: Progesterone;    Oocyte;    Inhibitory regulatory component (Ni);    Adenylyl cyclase;    Pertussis toxin;    N;    nucleotide binding regulatory component of adenylyl cyclases;    Ns stimulatory N;    Ni;    inhibitory N;    GMP-P(NH)P;    guanyl-5'-yl imidodiphosphate;    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(84)80562-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The possible susceptibility to pertussis toxin of the guanine nucleotide-dependent inhibition of oocyte adenylyl cyclase by progesterone was investigated. This action of progesterone is mediated by a membrane bound receptor as opposed to a receptor of cytosolic or nuclear localization. However, the inhibitory effect of progesterone was unaffected by pertussis toxin, even though the oocyte membrane N_i was fully ADP-ribosylated with pertussis toxin, as revealed by lack of further [³²P]ADP-ribosylation on subsequent re-incubation with pertussis toxin. These results indicate that the action of progesterone, in spite of being nucleotide-dependent, is either not mediated by N_i, suggesting the existence of an additional nucleotide regulatory component, or if mediated by N_i, involves a mode of regulation of this coupling protein that is different from that by which all other inhibitory hormones act on adenylyl cyclase.

【 授权许可】

Unknown