Localization of the amino acid substitution site in a fast migrating variant of human serum albumin

FEBS Letters

Minchiotti, L.¹  Galliano, M.¹  ladarola, P.¹ 

[1] Dipartimento di Biochimica, Via Taramelli 3B, Universitá, 27100 Pavia, Italy

关键词: Human serum albumin;    Genetic variant;    Tryptic peptide;    HPLC;    Amino acid sequence;    Point mutation;   

DOI  :  10.1016/0014-5793(85)80237-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Albumin Mi/Fg is an Italian genetic variant of human serum albumin arising from a Lys → Glu substitution which has been located in a CNBr fragment (CNBr VII) corresponding to the -COOH terminal portion of the molecule [(1984) J. Chromatogr. 298, 336-344]. Tryptic peptides of CNBr VII from normal and Mi/Fg albumin have been purified by reverse-phase high-performance liquid chromatography (RP-HPLC) and submitted to comparative structural studies. The amino acid sequence of the tryptic peptide of Mi/Fg variant that differs from the corresponding fragment of the normal serum albumin shows that the Lys → Glu substitution responsible for this variant is located at postion 573. This region of the albumin molecule is involved in the binding of long chain fatty acids.

【 授权许可】

Unknown