Albumin stabilizes 14,15‐leukotriene A4

FEBS Letters

Samuelsson, B.²  Rådmark, O.²  Fitzpatrick, F.¹  Haeggström, J.² 

[1] Department of Physiological Chemistry, Karolinska Institutet, S-104 01 Stockholm, Sweden

关键词: 14;    15-Oxido-5;    8;    10;    12-eicosatetraenoic acid;    14;    15-leukotriene A4;    Hydrolytic stability;    Bovine serum albumin;    Human serum albumin;    5;    6-LTA4;    5(S);    5;    6-oxido-7;    9-trans-11;    14-cis-eicosatetraenoic acid;    14;    15-LTA4;    14;    15-oxido-5;    8;    10;    12-eicosatetraenoic acid;    15-HPETE;    15-hydroperoxy-5;    8;    10;    13-eicosatetraenoic acid;    TXA2;    thromboxane A2;    PGI2;    prostacyclin;    HSA;    human serum albumin;    BSA;    bovine serum albumin;    HPLC;    high performance liquid chromatography;    RP—HPLC;    reversed phase high performance liquid chromatography;   

DOI  :  10.1016/0014-5793(83)80045-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

14,15-Leukotriene A₄ is a pivotal biosynthetic intermediate in 15-lipoxygenase initiated leukotriene biosynthesis. This compound hydrolyzes instantaneously in phosphate buffer at pH 7.4. However, addition of human or bovine albumin to otherwise identical buffer solutions increases its stability. Intact 14,15-leukotriene A₄ then decomposes by first-order kinetics with rate constants inversely proportional to the albumin concentration. Stabilization of 14,15-leukotriene A₄ under certain conditions may influence its proportionate transformation by enzymatic vs non-enzymatic processes.

【 授权许可】

Unknown