pp60 c‐srcis a substrate for phosphorylation when cells are stimulated to enter cycle

FEBS Letters

Friis, Robert R.¹  Tamura, Teruko¹  Bauer, Heinz¹ 

[1] Institut für Medizinische Virologie der Justus-Liebig-Universität Gießen, Frankfurter Str. 107, D-6300 Gießen, FRG

关键词: Protein kinase C;    pp60c-src;    Phosphorylation;    Cell cycle;    Oncogene;   

DOI  :  10.1016/0014-5793(84)81001-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The endogenous cellular oncogene products, pp60 ^c-src , exhibits a protein kinase activity, but is itself a phosphoprotein. Based on the assumption that pp60 ^c-src might play a role in the control of cell proliferation, we have studied its behaviour as a substrate for phosphorylation known to occur when quiescent, serum-deprived cells are stimulated to enter cell cycle following addition of either serum, platelet-derived growth factor or the phorbol ester derivative, 12-0-tetradecanoyl-phorbol-13-acetate. For this purpose a partial purification of pp60 ^c-src on DEAE ion-exchange chromatography was combined with immune precipitation. A 2–4-fold increase in serine phosphorylation of pp60 ^c-src was consistently observed after stimulation of quiescent cells to growth.

【 授权许可】

Unknown