FEBS Letters | |
pp60 c‐srcis a substrate for phosphorylation when cells are stimulated to enter cycle | |
Friis, Robert R.1  Tamura, Teruko1  Bauer, Heinz1  | |
[1] Institut für Medizinische Virologie der Justus-Liebig-Universität Gießen, Frankfurter Str. 107, D-6300 Gießen, FRG | |
关键词: Protein kinase C; pp60c-src; Phosphorylation; Cell cycle; Oncogene; | |
DOI : 10.1016/0014-5793(84)81001-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The endogenous cellular oncogene products, pp60 c-src , exhibits a protein kinase activity, but is itself a phosphoprotein. Based on the assumption that pp60 c-src might play a role in the control of cell proliferation, we have studied its behaviour as a substrate for phosphorylation known to occur when quiescent, serum-deprived cells are stimulated to enter cell cycle following addition of either serum, platelet-derived growth factor or the phorbol ester derivative, 12-0-tetradecanoyl-phorbol-13-acetate. For this purpose a partial purification of pp60 c-src on DEAE ion-exchange chromatography was combined with immune precipitation. A 2–4-fold increase in serine phosphorylation of pp60 c-src was consistently observed after stimulation of quiescent cells to growth.
【 授权许可】
Unknown
【 预 览 】
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RO201912020286073ZK.pdf | 490KB | download |