FEBS Letters | |
Calcium, calmodulin‐dependent protein phosphorylation in Neurospora crassa | |
Marme, Dieter1  Turian, Gilbert2  Ortega Perez, Ruben2  van Tuinen, Diederik2  | |
[1] Institut für Biologie III, Albert-Ludwigs-Universität, Schänzlestrasse 1, 7800 Freiburg i. Br., FRG;Laboratoire de Microbiologie Générale, Département de Biologie Végétale, Université de Genève, CH-1211 Geneva 4, Switzerland | |
关键词: Protein kinase; Calmodulin; Calcium; Neurospora crassa; | |
DOI : 10.1016/0014-5793(84)81187-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A calcium, calmodulin-dependent protein kinase activity has been partially purified by calmodulin-Sepharose affinity chromatography from the soluble fraction of Neurospora crassa. The phosphorylated peptide has an apparent molecular mass on SDS-polyacrylamide gel of 47 kDa. The apparent half maximal phosphorylation is obtained after 1.5 min at 30° C in the presence of calcium and calmodulin. The apparent half maximal activation of the phosphorylation is obtained at 1 μM calcium, and 0.1 or 0.2 μM calmodulin from bovine brain or Neurospora, respectively. The 32P incorporation is enhanced about 10-fold by calmodulin.
【 授权许可】
Unknown
【 预 览 】
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RO201912020286001ZK.pdf | 348KB | download |