Calcium, calmodulin‐dependent protein phosphorylation in Neurospora crassa

FEBS Letters

Marme, Dieter¹  Turian, Gilbert²  Ortega Perez, Ruben²  van Tuinen, Diederik² 

[1] Institut für Biologie III, Albert-Ludwigs-Universität, Schänzlestrasse 1, 7800 Freiburg i. Br., FRG;Laboratoire de Microbiologie Générale, Département de Biologie Végétale, Université de Genève, CH-1211 Geneva 4, Switzerland

关键词: Protein kinase;    Calmodulin;    Calcium;    Neurospora crassa;   

DOI  :  10.1016/0014-5793(84)81187-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A calcium, calmodulin-dependent protein kinase activity has been partially purified by calmodulin-Sepharose affinity chromatography from the soluble fraction of Neurospora crassa. The phosphorylated peptide has an apparent molecular mass on SDS-polyacrylamide gel of 47 kDa. The apparent half maximal phosphorylation is obtained after 1.5 min at 30° C in the presence of calcium and calmodulin. The apparent half maximal activation of the phosphorylation is obtained at 1 μM calcium, and 0.1 or 0.2 μM calmodulin from bovine brain or Neurospora, respectively. The ³²P incorporation is enhanced about 10-fold by calmodulin.

【 授权许可】

Unknown