Phytase production by fungi based on palm oil mill effluent

Humanosphere Science School 2017; 7th International Symposium for a Sustainable Humanosphere

地球科学;经济学

Sugiharto, A.^1

Research Center for Biology, Indonesian Institute of Sciences, Cibinong Science Center, Jl. Raya Bogor Km 46, Cibinong

16911, Indonesia^1

关键词: Aspergillus niger;    Enzyme production;    Media production;    Neurospora crassa;    Palm oil mill effluents;    Phytase production;    Randomized design;    Resistance to disease;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/166/1/012050/pdf
DOI  :  10.1088/1755-1315/166/1/012050

来源: IOP

【 摘 要 】

The utilization of phytase enzyme containing feed to monogastric and digastric livestock could increase the efficiency of nutrients uptake and livestock resistance to disease attacks. Palm oil mill effluent (POME) is one type of waste that has not been widely used in enzyme production. Some fungi that grow on POME indicate capable of producing phytase. The aims of this study is to utilize POME as a media production of phytase through fermentation by fungi with the eddition of C and N as sources. The experiment was arranged by complete randomized design with 5 replications. The result showed that isolated fungi from palm oil mill effluent (POME) was able to grow with pH value of 4 to 8. Furthermore, the two isolated fungi could optimally grow in pH value of 5 to 7. Through spores microscopic of the cross section of two fungi, the fungi were identified as Aspergillus niger and Neurospora crassa. Further study showed different results regarding on phytase enzyme activity of these two fungi in a modified media consists of 1% and 5% of POME with the addition of sucrose as the C source and 1% of peptone as the N source. The highest phytase production by Aspergillus niger was 0.393 U/mL substrate in media contain with 5% of POME and addition of 1% of peptone, for 96 hours and at 30°C. While for Neurospora crassa, the highest phytase production was 0.115 U/mL substrate, in media contain 1% of POME, 1% of sucrose, for 48 hours and at 30°C.