Isolation, Fractionation and Characterization of Catalase from Neurospora crassa (InaCC F226)

3rd International Seminar On Sciences "Sciences On Precision And Sustainable Agriculture"

Suryani^1 ; Ambarsari, L.^1 ; Lindawati, E.^1

Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Indonesia^1

关键词: Ammonium sulphate;    Catalase activity;    Neurospora crassa;    Optimum pH;    Optimum saturation;    Specific activity;    Total protein;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/58/1/012068/pdf
DOI  :  10.1088/1755-1315/58/1/012068

来源: IOP

【 摘 要 】

Catalase from Indigenous isolate Neurospora crassa InaCC F226 has been isolated, fractionated and characterized. Production of catalase by Neurospora crassa was done by using PDA medium (Potato Dextrosa Agar) and fractionated with ammonium sulphate with 20-80% saturation. Fraction 60% was optimum saturation of ammonium sulphate and had highest specific activity 3339.82 U/mg with purity 6.09 times, total protein 0.920 mg and yield 88.57%. The optimum pH and temperature for catalase activity were at 40°C and pH 7.0, respectively. The metal ions that stimulated catalase activity acted were Ca2+, Mn2+and Zn2+, and inhibitors were EDTA, Mg2+and Cu2+. Based on Kmand Vmaxvalues were 0.2384 mM and 13.3156 s/mM.