31P‐nuclear magnetic resonance studies of intact plasmodia of Physarum polycephalum

FEBS Letters

Yamada, Kazuhiro²  Tanokura, Masaru²  Kohama, Kazuhiro¹ 

[1] Department of Pharmacology, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;Department of Physiology, Medical College of Oita, Oita 879-56, Japan

关键词: 31P-NMR;    Intracellular pH;    ATP;    Mg2+;    Physarum polycephalum;    Actomyosin;   

DOI  :  10.1016/0014-5793(84)80932-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

³¹P-nuclear magnetic resonance spectra were obtained from intact plasmodial cells of Physarum polycephalum, where cytoplasmic streaming is generated by actin-myosin-ATP interaction. Several peaks were resolved and identified. They included ATP, ADP, orthophosphate and polyphosphates. Peaks for phosphocreatine, phosphoarginine or AMP were not detected. The intracellular pH and concentrations of ATP and free Mg²⁺ were estimated to be pH 6.9, 0.2–0.5 mM, and about 1 mM, respectively.

【 授权许可】

Unknown