A Wide Range of pH-Sensitive Fluorescent Probe based on Responsive Double Hydrophilic Block Copolymer for Real-Time Intracellular pH Sensing in Water Samples

7th Annual International Conference on Materials Science and Engineering

Zhou, Zijuan^1

School of Food Science and Nutrition, University of Leeds, Leeds

LS2 9JT, United Kingdom^1

关键词: Double-hydrophilic block copolymers;    Fluorescence intensities;    Fluorescent probes;    Intracellular pH;    PH measurements;    Qualitative measurements;    Red fluorescence;    Salicylaldoxime;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/562/1/012053/pdf
DOI  :  10.1088/1757-899X/562/1/012053

来源: IOP

【 摘 要 】

In this work, a wide range of pH-sensitive fluorescent probe has been designed by the responsive double hydrophilic block copolymer (DHBCs), which containing the fluorescein (Methacrylic salicylaldoxime, SHMa) and rhodamine (Rhodamine B methacryl hydrazide, RhSAMa) derivatives in the DHBCs. The pH-sensitive fluorescent probe gave blue and red fluorescence emissions at basic pH and acidic pH, respectively. Moreover, the fluorescent probe exhibited changes in fluorescence intensity with an alteration of turbidity of the block copolymer induced by temperature. Furthermore, we have developed a model that combines with the mobile phone for quantitative and qualitative measurement of pH value. The color extract APP for real-time pH measurement in mobile phone was constructed and used to detect the pH of the water samples.