Extended kinetic model of real-time polymerase chain reaction process

18th International Conference PhysicA.SPb

Fedorov, A.A.^1 ; Sochivko, D.G.^2 ; Varlamov, D.A.^3 ; Kurochkin, V.E.^1

Institute for Analytical Instrumentation, Russian Academy of Sciences, St.-Petersburg, Russia^1

Syntol JSC, Moscow, Russia^2

All-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, Moscow, Russia^3

关键词: Biomedical research;    Fluorescent probes;    Michaelis-Menten kinetic;    Probe hybridization;    Qualitative and quantitative analysis;    Real time polymerase chain reactions;    Real-time polymerase chain reaction (real-time PCR);    Temperature dependence;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/769/1/012019/pdf
DOI  :  10.1088/1742-6596/769/1/012019

来源: IOP

【 摘 要 】

Real-time polymerase chain reaction (real-time PCR) is the main molecular genetic method used for qualitative and quantitative analysis of specific nucleic acid sequences in many areas of biomedical research. Theoretical study of pCr models allows to estimate the influence of various reaction components and parameters, and to determine the unknown parameter values by approximating the experimental real-time PCR curves. An extended kinetic model of real-time PCR is presented. The model takes into account the enzyme activity based on Michaelis-Menten kinetics, the hybridization of complementary DNA fragments, the presence of a fluorescent probe used for detection of the reaction products, and the temperature dependence of primers and probe hybridization.