Competition between Li+ and Mg2+ for ATP in human erythrocytes A 31P NMR and optical spectroscopy study

FEBS Letters

Ramasamy, Ravichandran¹  de Freitas, Duarte Mota¹ 

[1] Department of Chemistry, Loyola University of Chicago, 6525 N. Sheridan Road, Chicago, IL 60626, USA

关键词: ATP;    Li+;    Mg2+;    Competition;    NMR;    31P-;   

DOI  :  10.1016/0014-5793(89)81197-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated the influence of Li⁺ on free intracellular Mg²⁺ concentration in human erythrocytes by ³¹P NMR and optical absorbance spectroscopies. In red cells loaded with 3 mM intracellular Li⁺, the chemical shift separation between the α- and β-phosphate resonances of MgATP²⁻ was approx. 0.9 ppm larger than that observed in Li⁺-free red cells. By analyzing the interaction of each red cell component with Mg²⁺ and Li⁺, we found that Mg²⁺ is displaced in part from MgATP²⁻ upon addition of Li⁺ and that the released Mg²⁺ is bound to the red cell membrane causing an overall decrease in free intracellular Mg²⁺ concentration.

【 授权许可】

Unknown