Changes in intracellular pH and inorganic phosphate concentration during and after muscle contraction as studied by time‐resolved 31P‐NMR

FEBS Letters

Tanokura, Masaru¹  Kazuhiro, Yamada¹ 

[1] Department of Physiology, Medical College of Oita, Hasama, Oita 879-56, Japan

关键词: 31P-NMR;    Intracellular pH;    Alkalinization;    Frog muscle;    Recovery metabolism;    Buffering power;   

DOI  :  10.1016/0014-5793(84)80480-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The changes in intracellular pH and concentration of inorganic phosphate (P_i) were studied during and after contraction of bullfrog sartorius muscles by using ³¹P-NMR. The intracellular pH was 7.04 in resting muscles at 4°C and an alkalinization by as much as 0.08 pH unit occurred following 5 s tetanic contraction. During the recovery period after contraction the increased level of intracellular pH returned to the resting level, the time course of which coincided with that of the recovery of the P_i concentration. Based on the changes in the intracellular pH and P_i concentration, the buffering power as estimated to be 18 mM/pH unit.

【 授权许可】

Unknown