Modulations of glycerophosphorylcholine and phosphorylcholine in Friend erythroleukemia cells upon in vitro‐induced erythroid differentiation: a 31P NMR study

FEBS Letters

Carpinelli, G.²  Podo, F.²  Proietti, E.¹  Belardelli, F.¹  Gessani, S.¹  Di Vito, M.² 

[1] Laboratorio di Virologia, Istituto Superiore di Sanità, Rome, Viale Regina Elena, 299, 00161 Rome, Italy;Laboratorio di Biologia Cellulare, Istituto Superiore di Sanità, Rome, Viale Regina Elena, 299, 00161 Rome, Italy

关键词: Friend erythroleukemia cell;    Erythroid differentiation;    31P NMR;    Glycerophosphorylcholine Phosphorylcholine;    Friend erythroleukemia cells;    FLC;    dimethylsulfoxide;    DMSO;    hexamethylenebisacetamide;    HMBA;    perchloric acid;    PCA;    glycerophosphorylcholine;    GroPCho;    glycerophosphorylethanolamine;    GroPEtn;    phosphorylcholine;    P-Cho;    phosphatidylcholine;    PtdCho;   

DOI  :  10.1016/0014-5793(84)80917-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A ³¹P NMR study has been carried out on Friend erythroleukemia cells (FLC) induced to undergo erythroid differentiation in vitro. Significant levels of glycerophosphorylcholine (GroPCho) and phosphorylcholine (P-Cho) were identified both in the untreated cells and in their PCA extracts. In FLC treated 4 days in vitro with either dimethylsulfoxide (DMSO) or hexamethylenebisacetamide (HMBA), the intracellular concentration of P-Cho was markedly increased, whereas that of GroPCho appeared to be significantly reduced. HMBA was more effective than DMSO in producing this effect. The concomitant modulations of GroPCho and P-Cho in differentiated FLC suggest the hypothesis that erythroid differentiation involves modifications of the regulatory mechanisms controlling biosynthesis and catabolism of phospholipids.

【 授权许可】

Unknown