Methylation of a middle repetitive DNA sequence class during differentiation in Friend erythroleukemia cells

FEBS Letters

Smith, Steven S.¹  Tolberg, Mary Ellen¹ 

[1] Department of Molecular Biology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010, USA

关键词: DNA methylation;    Friend erythroleukemia cell;    Hypomethylation of DNA;    Repetitive DNA sequence;    Induction of differentiation;    APT-cellulose;    aminophenylthioether cellulose;    DPT-cellulose;    diazophenyl-thioether cellu-lose;    HMBA;    hexamethylene-bis-acetamide;    HPLC;    high-performance liquid chromatography;    bp;    base pairs;    5-MCyt;    5-methylcytosine.;   

DOI  :  10.1016/0014-5793(84)80951-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have examined the methylation patterns within middle repetitive sequences in Friend erythroleukemia cells. Mouse-interspersed-family-1 (MIF-1) and a group characterized by a 1350-bp Eco-Bam fragment cloned into pBR322 as plasmid pFS-13, are both less modified in Friend cell DNA than in normal tissue DNA. The pattern of methylation present in pFS-13 homologous sequences was found to be stable during cell division, i.e., somatically inherited, and stable during differentiation induced by HMBA.

【 授权许可】

Unknown