Changes in chromatin structure due to hypomethylation induced with 5‐azacytidine or DL‐ethionine

FEBS Letters

Fajkus, Jiří¹  Bezděk, Milan¹  Vyskot, Boris¹ 

[1] Institute of Biophysics, Czechoslovak Academy of Sciences, CS-61265 Brno, Czechoslovakia

关键词: DNA methylation;    Nucleosome positioning;    Repetitive DNA sequence;    Tobacco tissue culture;    AzaC;    5-azacytidine;    EtBr;    ethidium bromide;    Ethi;    DL-ethionine;    MNase;    micrococcal nuclease;    TE;    10 mM Tris-HC1;    1 mM EDTA;    pH 8;   

DOI  :  10.1016/0014-5793(92)81449-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Changes in chromatin structure of the HRS60 family of repetitive sequences in tobacco DNA were studied after hypomethylation induced with 5-azacytidine or DL-ethionine. The TaqI site in the HRS60 units lies in nucleosomal core regions and its cleavage is enhanced in the hypomethylated chromatin. In contrast, the cleavage of the Sau3A1 site located in linker DNA does not depend on the level of methylation of DNA.

【 授权许可】

Unknown